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Abstract #3932

Compartmentation in the Myocardium: On the Fate of Exogenous Versus Glycolytically Derived Pyruvate

Colin Purmal 1 , Blanka Kucejova 1 , Shawn Burgess 1 , Craig Malloy 1 , Dean Sherry 1 , and Matthew E Merritt 1

1 AIRC, UTSW Medical Center, Dallas, TX, United States

Depending upon the underlying energetic demands for ATP production in the myocardium, pyruvate has multiple metabolic fates: exchanging with alanine or lactate, being oxidized via the pyruvate dehydrogenase (PDH) complex, or carboxylated to form oxaloacetate. Here the metabolism of exogenous hyperpolarized [1- 13 C]pyruvate is compared with that of glucose in the perfused mouse heart. A battery of analyses indicate that 1)exogenous pyruvate oxidation and glucose oxidation are not synonymous, 2)hyperpolarized lactate production does not necessarily report on [lactate] in the heart, and 3) propionate activated PDH flux even in the presence of a short chain fatty acid. The simplest interpretation of these phenomena requires a two compartment model of myocardial metabolism.

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