Meeting Banner
Abstract #3813

Sequential and Time-lapse MRI Monitoring of Peripheral Macrophage Recruitment and Migration in Mouse Brain

Yuki Mori 1,2 , Ting Chen 1 , Koji Ohno 3 , Shinichi Yoshida 4 , Yoshiyuki Tago 4 , Tetsuya Fujisawa 5 , Yuuto Kashiwagi 1 , Masaki Fukunaga 1,2 , Yutaka Komai 6 , Yutaka Hata 5 , and Yoshichika Yoshioka 1,2

1 Biofunctional Imaging, Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan, 2 Center for Information and Neural Networks (CiNet), National Institute of Information and Communications Technology (NICT) and Osaka University, Suita, Osaka, Japan, 3 Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan, 4 Frontier Biochemical and Medical Research Laboratories, Kaneka Corporation, Takasago, Hyogo, Japan, 5 Graduate School of Engineering, University of Hyogo, Himeji, Hyogo, Japan, 6 Single Molecule Imaging, IFReC, Osaka University, Suita, Osaka, Japan

Combination of MRI and nanoparticles has a possibility for visualizing the dynamics of cells in mouse brain. In this study, we focused on peripheral macrophages and attempted to track them noninvasively. We also attempted to monitor dynamic behaviors of cell migration with time-lapse MRI movie. MRI can successfully monitor the recruitment of peripheral macrophages into CNS even in normal as well as abnormal condition such as systemic inflammation or brain ischemia. Time-lapse MRI movie may reveal critical insights into cell behaviors that are not readily evident by microscopy. Our technique could contribute to reveal the mechanisms of neuro-immune crosstalk.

This abstract and the presentation materials are available to members only; a login is required.

Join Here