Abstract #3804
Ascorbic Acid Enhances Pyruvate Dehydrogenate Flux In Isolated Perfused Rat Lungs
Hoora Shaghaghi 1 , Stephen J. Kadlecek 1 , Sarmad Siddiqui 1 , Mehrdad Pourfathi 1 , Profka Harrilla 1 , and Rahim R. Rizi 1
1
Radiology, University of Pennsylvania,
Philadelphia, Pennsylvania, United States
Ascorbic acid is a first line pulmonary defense
mechanism against endogenous and exogenous oxygen
reactive species. As such, we investigated the effect of
ascorbate on lung metabolic activity using
hyperpolarized [1-13C] pyruvate. The bicarbonate signal
significantly increased in the presence of ascorbate.
Addition of
N,N,N′,N′-tetramethyl-p-phenylenediamine(TMPD), a
well-known compound used in electron transfer chain
investigations, into the ascorbate-containing perfusate
amplifies this enhancement. Since the reaction rate of
ascorbate plus TMPD is 30 times faster than that of
ascorbate alone, we concluded that the effect of
ascorbate on PDH flux is a mitochondrial membrane linked
effect.
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