Abstract #3602
In vivo assessment of skeletal muscle ATP synthesis in Ob/Ob mice
Patricia Maria Nunes 1 , Barbara Janssen 1 , Andor Veltien 1 , Henk Arnts 2 , Cees Tack 3 , and Arend Heeschap 1
1
Radiology, Radboud University Nijmegen
Medical Centre, Nijmegen, Gelderland, Netherlands,
2
Central
Animal Laboratory, Radboud University Nijmegen Medical
Centre, Nijmegen, Gelderland, Netherlands,
3
Internal
Medicine, Radboud University Nijmegen Medical Centre,
Nijmegen, Gelderland, Netherlands
Caloric overload stresses energy homeostasis maintenance
in key metabolic tissues as the skeletal muscle. This
phenomenon is, in the long run, characterized by
intramyocellular lipid accumulation and impaired
oxidative capacity. We tested in leptin deficient mice
(Ob/Ob) mice whether ATP synthesis was impaired, by
means of
31
P
MRS. The overall muscular Pi
ATP
flux assessed with ST showed no differences between WT
and Ob/Ob mice, despite greater Pi concentration. When
stimulated, muscles of Ob/Ob mice produced lower force
and were prone to fatigue. Preliminary data suggests
that Ob/Ob mice have higher mitochondrial ATP synthesis,
as alluded by the PCr recovery curves.
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