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Abstract #2934

Metabolic reprograming in IDH mutant glioma cells

Jose Luis Izquierdo Garcia 1 , Pia Eriksson 1 , Myriam Chaumeil 1 , Russell O Pieper 2 , Joanna J Phillips 2 , and Sabrina M Ronen 1

1 Radiology, UCSF, San Francisco, CA, United States, 2 Neurological Surgery, Helen Diller Research Center, UCSF, San Francisco, CA, United States

This study demostrates that the activity of mutant IDH1 results not only in production of the oncometabolite 2-HG, but also in a wider metabolic reprogramming. Two cell models were investigated by 1H-MRS: the U87-based and the E6/E7/hTERT Normal Human Astrocyte (NHA)-based models. Metabolic profiling discriminated between wild-type and mutant IDH cells. A significant drop in the concentration of acetate, aspartate, glutamine and myo-inositol was observed in U87IDHmut cells and lower creatine and lactate concentration was observed in NHAIDHmut cells. In both models, IDHmut cells showed a lower concentration of glutamate and phosphocholine as well as the expected elevation in 2-HG

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