Abstract #2932
Electron paramagnetic resonance spectroscopy for in vivo measurement of tumour extracellular pH- the effect of X-ray irradiation
Jonathan Goodwin 1,2 , Katsuya Yachi 3 , Masaki Nagane 4 , Hironobu Yasui 4 , Yusuke Miyaki 3 , Osamu Inanami 4 , Andrey Bobko 5 , Valery Khramstov 5 , and Hiroshi Hirata 3
1
Department of Radiology, Hokkaido University
Hospital, Sapporo, Hokkaido, Japan,
2
Division
of Ultrahigh Field MRI, Iwate Medical University, Yahaba,
Iwate, Japan,
3
Division of Bioengineering and
Bioinformatics, Hokkaido University, Sapporo, Japan,
4
Laboratory
of Radiation Biology, Hokkaido University, Sapporo,
Japan,
5
Division
of Pulmonary, Allergy, Critical Care & Sleep Medicine,
The Ohio State University, Columbus, Ohio, United States
Accurate measurement of extra cellular pH (pHe) in
tumour may offer valuable information regarding cell
viability and therapeutic response. In this work, 750
MHz CW-EPR spectroscopy was used to assess pHe of C3H
HeJ mice hind leg squamous cell tumour after
intra-venous tail vein injection during stages of normal
tumour growth, and in response to a single 10 Gy dose of
X-ray irradiation. Through measurement of the hyperfine
coupling constant, an inverse relationship was observed
between tumour pHe and volume, with an accuracy of less
than 0.1pH units. This relationship was disrupted by a
single 10 Gy radiotherapy dose.
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