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Abstract #2932

Electron paramagnetic resonance spectroscopy for in vivo measurement of tumour extracellular pH- the effect of X-ray irradiation

Jonathan Goodwin 1,2 , Katsuya Yachi 3 , Masaki Nagane 4 , Hironobu Yasui 4 , Yusuke Miyaki 3 , Osamu Inanami 4 , Andrey Bobko 5 , Valery Khramstov 5 , and Hiroshi Hirata 3

1 Department of Radiology, Hokkaido University Hospital, Sapporo, Hokkaido, Japan, 2 Division of Ultrahigh Field MRI, Iwate Medical University, Yahaba, Iwate, Japan, 3 Division of Bioengineering and Bioinformatics, Hokkaido University, Sapporo, Japan, 4 Laboratory of Radiation Biology, Hokkaido University, Sapporo, Japan, 5 Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, The Ohio State University, Columbus, Ohio, United States

Accurate measurement of extra cellular pH (pHe) in tumour may offer valuable information regarding cell viability and therapeutic response. In this work, 750 MHz CW-EPR spectroscopy was used to assess pHe of C3H HeJ mice hind leg squamous cell tumour after intra-venous tail vein injection during stages of normal tumour growth, and in response to a single 10 Gy dose of X-ray irradiation. Through measurement of the hyperfine coupling constant, an inverse relationship was observed between tumour pHe and volume, with an accuracy of less than 0.1pH units. This relationship was disrupted by a single 10 Gy radiotherapy dose.

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