Meeting Banner
Abstract #2822

Combining fMRI with optical Ca 2+ recordings to define the impact of brain states on BOLD

Miriam Schwalm 1 , Lydia Wachsmuth 2 , Florian Schmid 2 , Cornelius Faber 2 , and Albrecht Stroh 1

1 Institute of Microscopic Anatomy & Neurobiology - Research Group Molecular Imaging & Optogenetics, Johannes Gutenberg-University Mainz, Mainz, Rheinland-Pfalz, Germany, 2 Department of Clinical Radiology, University of Mnster, Nordrhein-Westfalen, Germany

Revealing the neuronal underpinnings of fMRI requires monitoring of suprathreshold spiking. We implemented the combination of optical Ca2+ recordings with BOLD fMRI in rat somatosensory cortex and recorded Ca2+ and BOLD signals in two brain states: in Up/Down state, sensory stimulation did not lead to BOLD activation, despite initiation of Ca2+ waves. In persistent Up state, we recorded primary neuronal Ca2+ responses, and BOLD activation. The duration of the BOLD response depended on the duration of stimulus trains, even though Ca2+ transients revealed adaptation of the neuronal response. Our data suggests that neuronal spiking plays a minor role in BOLD.

This abstract and the presentation materials are available to members only; a login is required.

Join Here