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Abstract #2793

Detection of metabolic changes in SCC tumor by mTOR inhibition using hyperpolarized 13 C-pyruvate MRI

Keita Saito 1 , Shingo Matsumoto 1 , Yoichi Takakusagi 1 , Masayuki Matsuo 1 , H Douglas Morris 2 , Hellmut Merkle 2 , Martin J Lizak 2 , Jeeva P Munasinghe 2 , Nallathamby Devasahayam 1 , Sankaran Subramanian 1 , James B Mitchell 1 , and Murali C Krishna 1

1 Radiation Biology Branch, National Cancer Institute, Bethesda, Maryland, United States, 2 National Institute of Neurological Disorder and Stroke, Bethesda, Maryland, United States

We investigated effects of an mTOR inhibitor rapamycin on pyruvate metabolism in squamous cell carcinoma (SCC) implanted in mice leg using 13 C-MRI with hyperpolarized [1- 13 C]pyruvate. The signal of [1- 13 C]pyruvate and [1- 13 C]lactate were detected in SCC tumor immediately after hyperpolarized [1- 13 C]pyruvate injection. [1- 13 C]lactate to [1- 13 C]pyruvate ratio (Lac/Pyr) in the SCC tumors increased as tumor grew in non-treated control mice, whereas it significantly dropped after 2 days of rapamycin treatment. So, mTOR inhibition causes decrease of LDH activity in SCC tumor, and lactate to pyruvate ratio monitored using hyperpolarized 13 C-MRI would become a useful marker for tumor response to therapy.

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