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Abstract #2775

Measurements and Modeling of Hyperpolarized 1-13C in the Perfused Lung in Relation to Organ Redox State

Stephen J. Kadlecek 1 , Hoora Shaghaghi 1 , Mehrdad Pourfathi 1 , Sarmad Siddiqui 1 , Jennia Rajaei 1 , Profka Harrilla 1 , and Rahim R. Rizi 1

1 Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, United States

We present the measurements of lactate pool labeling rates in the isolated, perfused rat lung using hyperpolarized 1-13C pyruvate. The rates are shown to vary strongly with perfusate constituents (unpolarized lactate and pyruvate, as well as hyperpolarized pyruvate concentration and can be accurately reproduced using a three-compartment model which incorporates previously measured transport and enzymatic activity measurements, as well as concentrations of enzyme and cofactors. We conclude that intracellular conditions (e.g., redox state) are strongly perturbed during a typical hyperpolarized pyruvate measurement, and that consideration of metabolic flux through LDH is not necessary to reproduce experimental results.

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