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Abstract #1085

Metabolic Analysis of Slowly Cycling Melanoma Cells

Sergey Magnitsky 1 and Geetha Mohan 1

1 Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, California, United States

Low efficacy of existing treatment for various human cancers has been attributed to the existence of cancer stem cells. Such slowly cycling sub-population of cells in melanoma exhibits high tumorigenicity, self-renewal capacity and drug resistance. The goal of this study was to delineate metabolic differences between slowly cycling sub-population and bulk tumor cells. Ethanol/chloroform cells extracts were prepared from sorted cells. High-resolution NMR spectra were acquired. NMR data revealed high intracellular lactate and choline concentration in slowly cycling sub-population cells compared to fast cycling tumor cells. Approximately 70% of lactate produced in unsorted cells was produced by slowly cycling sub-population.

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