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Abstract #1082

Metabolic profile of human breast tissues studied by in-vitro NMR spectroscopy

Naranamangalam R Jagannathan 1 , Rani G Sah 1 , Uma Sharma 1 , Rajinder Parshad 2 , and Vurthaluru Seenu 2

1 Department of NMR & MRI Facility, All India Institute of Medical Sciences, New Delhi, Delhi, India, 2 Department of Surgical Disciplines, All India Institute of Medical Sciences, New Delhi, Delhi, India

High-resolution in-vitro NMR spectroscopy was used to determine the absolute concentration of various metabolites in 23 breast tissues, both involved (n=11) and non-involved (n=13). One-dimensional and two- dimensional NMR experiments (DQF-COSY and TOCSY) were carried out at 400.13 MHz. The concentration of Lactate (14.0 11.8 mmol/kg), Creatine (1.9 1.2 mmol/kg), Choline (3.1 2.2 mmol/kg), Glycerophosphocholine/Phosphocholine (5.2 4.1 mmol/kg) and Glutamate/Glutamine (8.3 5.4) mmol/kg in involved tissues were significantly higher compared to non-involved tissues, indicating a general increase in the metabolic activity in tumor tissues and thus providing an insight into the tumor metabolism.

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