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Abstract #1081

NMR spectroscopy identifies two subtypes with different metabolic profiles in stem-like cells from Glioblastoma multiforme

Alessandra Palma 1 , Sveva Grande 1 , Anna Maria Luciani 1 , Antonella Rosi 1 , Mauro Biffoni 2 , Lucia Ricci-Vitiani 2 , Daniele Runci 2 , Roberto Pallini 3 , Vincenza Viti 4 , and Laura Guidoni 4

1 Dipartimento di Tecnologie e Salute, Istituto Superiore di Sanit and INFN Sanit Group, Roma, Italy, Italy, 2 Dipartimento di Ematologia, Oncologia e Medicina Molecolare, Istituto Superiore di Sanit, Italy, Italy, 3 Dipartimento di Neurochirurgia, Universit Cattolica del Sacro Cuore, Roma, Italy, Italy, 4 INFN Sanit Group, Roma, Italy, Italy

Recurrence of the glioblastoma after conventional treatments is attributed to the overgrowth of stem-like cancer cells resistant to treatments. 1H NMR spectra run on glioblastoma derived stem-like cells grown in neurospheres showed intense signals common in brain and brain tumours including NAA, creatine, myoinositol, glutamine and neutral lipids. Unsupervised cluster analysis performed on spectral data of cells from 27 glioblastoma patients identified two different NMR-based groups with different metabolism. A mixed neuralastrocyte metabolic phenotype with a strong neuronal fingerprint prevailed in one group (15 cell lines) and an astrocytic/glioma-like metabolism was prevalent in the other cluster (12 cell lines).

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