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Abstract #0805

Identifying Intracellular Sperm Metabolites Using HR MAS NMR

Jack Tristan Pearson 1 , Steven Reynolds 2 , Adriana Bucur 2 , Allan Pacey 1 , and Martyn Paley 2

1 Human Metabolism, The University of Sheffield, Sheffield, South Yorkshire, United Kingdom, 2 Cardiovascular Science, The University of Sheffield, Sheffield, South Yorkshire, United Kingdom

Approximately 25% of males have poor semen quality and diagnosis of male infertility relies on techniques developed in the 1950s. To investigate sperm metabolism, HR MAS NMR of boar sperm was performed at two incubation temperatures and in the presence or absence of α-chlorohydrin (an inhibitor of glycolysis). HR MAS successfully identified significant increases in acetate, glutamine and glucose at increased incubation temperatures. A significant decrease in lactate was identified when metabolism was inhibited with α-chlorohydrin. This data supports the potential use for HR MAS in identification of metabolic biomarkers in sperm and for infertility diagnosis in the clinical setting.

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