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Abstract #0769

Overhauser-enhanced MRI as a non invasive probe of BBB breakdown and redox state following ischemia/reperfusion

Matthew S. Rosen 1,2 , Mathieu Sarracanie 1,2 , Brandon D. Armstrong 1,2 , Fanny Herisson 3,4 , Najat Salameh 1,5 , and Cenk Ayata 3,4

1 Department of Radiology, MGH/Athinoula A. Martinos Center for Biomedical Imaging, Charlestown, MA, United States, 2 Department of Physics, Harvard University, Cambridge, MA, United States, 3 Department of Radiology, MGH/Neurovascular Research Lab, Charlestown, MA, United States, 4 Department of Radiology, Harvard Medical School, Boston, MA, United States, 5 Institut de Physique des Systmes Biologiques, EPFL, Lausanne, Vaud, Switzerland

A new method to probe hyperacute BBB breakdown following ischemic stroke in a rodent model using Overhauser-MRI (OMRI) in conjunction with an injected stable free radical, TEMPOL, is described. We present OMRI images of TEMPOL crossing the BBB following ischemia/reperfusion in vivo. The use of OMRI in conjunction with TEMPOL as an exogenous imaging agent for stroke is a new and novel approach, and suggests that TEMPOL may be a suitable probe for observing early BBB breakdown following reperfu-sion in rodent I/R models. We hypothesize that temporally resolved OMRI may be used to indicate the redox status of ischemic tissue.

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