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Abstract #0533

Glutamine is the main source of 2-HG production in IDH1 mutant glioma cells

Jose Luis Izquierdo Garcia 1 , Pia Eriksson 1 , Cai Larry 1 , Myriam Chaumeil 1 , Russell O Pieper 2 , Joanna J Phillips 2 , and Sabrina M Ronen 1

1 Radiology, UCSF, San Francisco, CA, United States, 2 Neurological Surgery, Helen Diller Research Center, UCSF, San Francisco, CA, United States

Mutations in Isocitrate dehydrogenase (IDH1) have been reported in over 70% of low-grade gliomas and secondary glioblastomas (GBM) and they are associated with the accumulation of the oncometabolite 2-HG within the tumor. Despite these observations, the metabolic fluxes associated with 2-HG production are not fully understood. This study demonstrates using 13C MRS in combination 13C-labeled glucose and glutamine that glucose does not contribute significantly to 2-HG production and that glutamine is the main source of 2-HG in our U87 GBM mutant IDH1 cells.

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