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Abstract #0477

Detection of transgene expression using hyperpolarized 13 C urea and diffusion-weighted MRS

P. Stephen Patrick 1 , Mikko I. Kettunen 1,2 , Sui-Seng Tee 1 , Tiago B. Rodrigues 1 , Eva M. Serrao 1 , Kerstin Timm 1 , and Kevin M. Brindle 1

1 CRUK Cambridge Institute and Department of Biochemistry, University of Cambridge, Cambridge, Cambridgeshire, United Kingdom, 2 A.I.Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland

Diffusion spectroscopy of hyperpolarized [ 13 C]urea was used to assess urea transporter expression as a potential gene reporter in vivo . Significantly decreased apparent diffusion coefficient was measured for urea transporter expressing tumors compared to their non-expressing controls. No differences in tumor cellularity were observed. The results suggest that expression of transporter in combination with diffusion spectroscopy might be used as another approach for detecting gene reporter expression.

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