Abstract #0201
Simultaneous detection of multiple metal ions using a single 19 F-iCEST probe
Amnon Bar-Shir 1,2 , Nirbhay N Yadav 1,3 , Assaf A Gilad 1,2 , Peter CM van Zijl 1,3 , Michael T McMahon 1,3 , and Jeff WM Bulte 1,2
1
Russell H. Morgan Department of Radiology
and Radiological Science, the Johns Hopkins University
School of Medicine, Baltimore, MD, United States,
2
Cellular
Imaging Section, Institute for Cell Engineering, the
Johns Hopkins University School of Medicine, Baltimore,
MD, United States,
3
F.M.
Kirby Research Center for Functional Brain Imaging,
Kennedy Krieger Institute, Baltimore, MD, United States
Metal ion levels in biological systems have been
extensively studied using optical probes. However,
specific detection of low levels of metal ions
in
vivo
using non-invasive methodologies remains a
formidable challenge. We present an approach for
specifically sensing the presence of Zn
2+
and
Fe
2+
using
the single fluorinated chelate TF-BAPTA. By exploiting
the dynamic exchange between the ion-bound and free
TF-BAPTA, and capitalizing on the different chemical
shifts of
19
F
upon binding of Zn
2+
or
Fe
2+
, we were able to detect both ions
simultaneously using ion CEST (iCEST)
19
F
MRI.
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