Abstract #0194
In vivo Overhauser-enhanced MRI of Proteolytic Activity
Neha Koonjoo 1 , Elodie Parzy 1 , Philippe Massot 1 , Matthieu Lepetit-Coiff 1,2 , Sylvain R.A Marque 3 , Jean-Michel Franconi 1 , Eric Thiaudiere 1 , and Philippe Mellet 1,4
1
Centre de Rsonance Magntique des Systmes
Biologiques, UMR 5536, CNRS, University Bordeaux Segalen,
Bordeaux, France, Metropolitan,
2
Siemens
France, Saint-Denis, France, Metropolitan,
3
UMR
7273 Aix-Marseille Universit, Marseille, France,
Metropolitan,
4
University
Bordeaux Segalen, INSERM, France, Metropolitan
Proteolysis of a nitroxide-labeled macromolecule was
detected in the intestinal tract of living mice by
Overhauser-enhanced MRI. Using an Overhauser-effect
switch, namely a nitroxide with an unpaired electron
bound to the macromolecule, here elastin, Overhauser
enhancement was generated only after the protein carrier
was digested. A high signal enhancement indicated
elastin proteolysis by pancreatic elastase secreted in
the duodenum. Highly resolved 3D keyhole OMRI images of
resolution 0.5mm
3 were acquired with a fully
balanced steady state sequence TrueFISP at 0.2T (Magnetom
Open Viva Siemens) in 18 seconds. This current research
is a step towards in vivo pathology-related proteolysis
detection.
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