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Abstract #3970

Studying magnetic susceptibility, microstructural compartmentalisation and chemical exchange in a formalin-fixed, whole-brain specimen

Kwok-Shing Chan1, Jeroen Mollink2, Jenni Schulz1, Anne-Marie van Cappellen van Walsum2, and José P. Marques1
1Donders Institute for Brain, Cognition and Behaviour, Radboud University, Nijmegen, Netherlands, 2Department of Medical Imaging, Anatomy, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, Nijmegen, Netherlands

In this work, we study the feasibility of using formalin-fixed post-mortem specimen to investigate MRI phase contrast in brain tissue. Despite the magnetic susceptibility maps derived from in vivo and ex vivo imaging share similar contrasts, the non-susceptibility contributions on measured fields of ex vivo sample were clear affected by fixation process, and we found its microstructural compartmentalisation effect are also greatly reduced. Therefore, extra care has to be taken to directly translate ex vivo findings to in vivo applications.

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