Abstract #1223

Investigating differences in lung cancer cells with induced and de-induced cisplatin resistance by 1H HR-MAS NMR metabolomics

Martina Vermathen¹, Hendrik von Tengg-Kobligk^2,3, Martin Nils Hungerbühler^2,3, Christoph Kempf^2,3, Peter Vermathen^2,3, and Nico Ruprecht^2,3

¹Department of Chemistry and Biochemistry, University Bern, Bern, Switzerland, ²University Institute of Diagnostic, Interventional and Pediatric Radiology, University Bern, Bern, Switzerland, ³Department of BioMedical Research, University Bern, Bern, Switzerland

Cisplatin (cisPt) is an important drug that is used against various cancers, including advanced lung cancer. However, drug resistance is a major problem. Here, a high resolution magic angle spinning (HR-MAS) NMR study is presented determining the metabolic profile of lung cancer cells (A24) and metabolic adaptations in different levels of induced cisPt-resistance as well as in their de-induced counterparts. More than 40 metabolites were identified. Metabolic adaptations were determined, which increased with higher cisPt-resistance. Importantly, de-induced cell lines demonstrated similar metabolic adaptations. Metabolites predominantly changed in cisPt resistant cells and their de-induced counterparts include glutathione or taurine.

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