Abstract #0931

Metabolomic Characterization of renal cell carcinoma patient-derived xenografts and derived Tissue Slice Cultures

Deepti Upadhyay¹, Jinny Sun¹, Joao Piraquive Agudelo¹, Hongjuan Zhao², Rosalie Nolley², Robert Bok¹, James D. Brooks², Donna M. Peehl¹, John Kurhanewicz¹, and Renuka Sriram¹

¹Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA, United States, ²Department of Urology, Stanford University, Stanford, CA, United States

NMR based stable isotope resolved metabolomics was used for the metabolic characterization of renal cell carcinoma (RCC) patient-derived xenografts (PDXs) and their derived tissue slice cultures (TSCs). Targeted metabolomics and [U-¹³C] glucose-labeling studies indicate metabolic heterogeneity among PDXs of various pathologic and clinical stages. Both PDXs and TSCs exhibit high glycolytic rate and low TCA activity characteristic of RCC. However, there is variability in glycolytic rate and TCA activity among different PDXs and their corresponding TSCs.

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