Tractography is regularly used in neuroscientific research and neurosurgical planning for its ability to delineate fibre bundles non-invasively. The construction and analysis of tractograms often requires user-defined parameter choices such as streamline density thresholding for estimating tract volume. In this study, we assessed the reproducibility of a manual tractography pipeline that is applied in neurosurgical planning for oncology and epilepsy operations, and quantified the effect of streamline density thresholding on reproducibility. The pipeline was found to be robust in terms of reproducibility over the range of investigated thresholding levels with some variability between tracts.
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