Abstract #3170

A method to remove the influence of fixative concentration on post-mortem T2 maps using a kinetic-tensor model

Feng Qi¹, Karla Miller¹, Sean Foxley², Menuka Pallebage-Gamarallage³, Ricarda AL Menke¹, Olaf Ansorge³, Samuel A Hurley⁴, and Benjamin C Tendler¹

¹Wellcome Centre for Integrative Neuroimaging, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom, ²Department of Radiology, University of Chicago, Chicago, IL, United States, ³Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom, ⁴School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, United States

Postmortem imaging allows for validation of the origins of image contrast through comparisons with histology. However, the inclusion of formalin fixative substantially reduces the T₂. This reduction is (approximately) linear with concentration. Prior to scanning, samples are often placed in a fluid that has more favourable properties for imaging (e.g. perfluorocarbon fluids). This may lead to an outflow of fixative and an increase in T₂ at the tissue surface. Here we propose to correct for T₂ inhomogeneity by modelling the outflow of fixative within whole, human brains using a novel kinetic tensor model, which incorporates the effects of diffusion anisotropy.

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