Abstract #2042

Kinetics of O-17-labeled water in the brain parenchyma and cerebrospinal fluid of aquaporin-4-knockout mice on indirect MRI

Yutaka Hoshino¹, Hiroyuki Kameda¹, Taisuke Harada¹, Yuji Komaki², Tomoe Ishikawa³, Masato Yasui³, and Kohsuke Kudo¹

¹Department of Diagnostic and Interventional Radiology, Hokkaido University Hospital, Sapporo, Japan, ²Live Imaging Center, Central Institute for Experimental Animals, Kawasaki, Japan, ³Department of Pharmacology, Keio University School of Medicine, Tokyo, Japan

We evaluated the water kinetics of the brain of intoxication model mice to compare aquaporin-4-knockout (AQP4-KO) and wild-type (WT) control mice, using T2-weighted indirect MRI with ¹⁷O-labeled water. Eleven AQP4-KO and eight WT mice were scanned with 7T MRI using the RARE sequence. ¹⁷O-labeled water was administered intraperitoneally. The whole scanning took 540 phases and 45 min, and signals were converted to ¹⁷O concentrations. In ROIs of the brain parenchyma, steady-state ¹⁷O concentrations were slightly higher in AQP4-KO than in WT. The latest imaging method revealed that the deficiency of AQP4 might lead to water accumulation in the paravascular space.

This abstract and the presentation materials are available to members only; a login is required.

Join Here