Abstract #0497

The CLARITY procedure of lipid removal from brain tissue sample reveals the lipid-origin of MT contrast in CEST imaging experiment

Anna Orzylowska¹, Tymoteusz Słowik², Agata Chudzik¹, Anna Pankowska³, Wilfred W Lam⁴, and Greg J Stanisz^1,4,5

¹Department of Neurosurgery and Paediatric Neurosurgery, Medical University of Lublin, Lublin, Poland, ²Center of Experimental Medicine, Medical University of Lublin, Lublin, Poland, ³Department of Radiography, Medical University of Lublin, Lublin, Poland, ⁴Physical Sciences, Sunnybrook Research Institute, Toronto, ON, Canada, ⁵Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada

The study compares the differences between Z-spectra derived from CEST imaging of rat brain in vivo and after post-mortem CLARITY lipids removal procedure. The lipids removal nulled-out MT macromolecular-originating signal measured with B₁ saturation amplitudes of 3 and 5 µT as compared to in vivo, and resulted in negligible MT contribution to CEST Z-spectra acquired with B₁s of 0.5 and 0.75 µT, as opposite to living tissue, where the MT effect was significant. Our results showed that the macromolecular MT contribution into in vivo Z-spectra originates mostly from lipids, since the CLARITY technique removed the MT component from the spectrum.

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