Abstract #0272

Mapping of exogenous choline uptake in brain tumors in vivo using Deuterium Metabolic Imaging (DMI)

Kevan L. Ip¹, Monique A. Thomas¹, Akshay Khunte¹, Kevin L. Behar², Robin A. de Graaf¹, and Henk M. De Feyter¹

¹Dept. of Radiology and Biomedical Imaging, Yale University, New Haven, CT, United States, ²Dept. of Psychiatry, Yale University, New Haven, CT, United States

A high level of intracellular choline is an established marker of malignancy in brain tumors. Here we investigate the uptake of exogenous choline in vitro using high resolution ¹H NMR into rodent glioblastoma cell lines. To map bloodborne uptake in vivo, we used the novel technique Deuterium Metabolic Imaging (DMI), combined with intravenous infusion of [²H₉]-choline in two orthotopic rat (RG2) and mouse (GL261) models of glioblastoma. DMI-based metabolic maps revealed high uptake of choline in the tumors, in a stark image contrast with normal-appearing brain, illustrating the potential of [²H₉]-choline chloride as metabolic imaging agent.

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