Abstract #0270

Deuterium MRI imaging of xenografted tumors following in vivo deuterated water labeling

Julian C. Assman¹, Jeffrey R. Brender², Don E. Farthing¹, Keita Saito², Kathrynne A. Warrick¹, Natella Maglakelidze¹, Hellmut R. Merkle³, Murali C. Krishna², Ronald E. Gress¹, and Nataliya P. Buxbaum¹

¹Experimental Transplantation and Immunotherapy Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, MD, United States, ²Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, MD, United States, ³Laboratory for Functional and Molecular Imaging, National Institute of Neurological Disorders and StrokNational Institutes of Health, Bethesda, MD, MD, United States

Water is a substrate for many biochemical reactions. If D₂O is ingested, it will be incorporated into proliferating cells. We hypothesized that rapidly proliferating cancer cells would become preferentially labeled with ²H which would allow visualization by deuterium MRI following a short in vivo D₂O labeling period. We initiated systemic D₂O labeling in HT-29 and MiaPaCa-2 xenograft models and performed deuterium MRI following 7 and 14 days of in vivo tumor growth and labeling. We show that small tumors could be distinguished from normal tissue by the incorporation D₂O into lipids with a greater sensitivity and selectivity than anatomical MRI.

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