TAT-functionalized perfluorocarbon nanoemulsion for 19F imaging of CAR T cells in vivo.
Dina Vinoo Hingorani1, Fanny Chapelin2, Emma Stares1, Stephen R Adams3, Hideho Okada4, and Eric T Ahrens1
1Radiology, University of California San Diego, La Jolla, CA, United States, 2Bioengineering, University of California San Diego, La Jolla, CA, United States, 3Pharmacology, University of California San Diego, La Jolla, CA, United States, 4Neurological Surgery, University of California San Francisco, San Francisco, CA, United States
To improve the utility of 19F MRI to detect lymphocytes in vivo, we looked into strategies to increase cell loading via the use of cell penetrating peptides as a component of perfluorocarbon (PFC) nanoemulsion formulation. We show an 8-fold increase in cell uptake without viability impairment. This TAT-PFC formulation can be used to provide insight into the modes of action of engineered T cell immunotherapy against cancer.
This abstract and the presentation materials are available to members only;
a login is required.
