Abstract #4017

A Potentially New Class of Redox-Sensitive CEST Agents: Preliminary Thiol-CEST Studies with Glutathione and Cysteine

Johnny Chen¹, Nirbhay N. Yadav^2,3, Abhishek Gupta¹, Tim Stait-Gardner¹, William S. Price¹, and Gang Zheng¹

¹School of Science and Health, Western Sydney University, Sydney, Australia, ²Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United States, ³F. M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, United States

In this study, we show that thiol-water proton exchange can generate MRI contrast by performing proton chemical exchange saturation transfer (¹H-CEST) experiments on glutathione (GSH) and cysteine (Cys). The thiol proton exchange was quantified at various pHs, with Cys thiol exhibiting faster and more base-catalyzed exchange than GSH. However, both GSH and Cys thiol exchange were too fast to generate contrast at physiological pH. Potential applications of thiol compounds as redox-sensitive CEST agents are suggested.

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