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Abstract #3198

Imaging the evolution acute fear to anxiety: Longitudinal whole brain imaging in living mice of neural activity with MEMRI

Elaine L Bearer1,2, Daniel Barto3, and Rusell E Jacobs4

1Elaine Bearer, University of New Mexico Health Sciences Center, Albuquerque, NM, United States, 2Biology, California Institute of Technology, Pasadean, CA, United States, 3Pathology, University of New Mexico Health Sciences Center, Albuquerque, NM, United States, 4Physiology, Zilkha Neurogenetic Institute USC, Los Angeles, CA, United States

Life-threatening events cause extreme fear, which evolves in vulnerable people into a debilitating mental illness--post-traumatic stress disorder (PTSD). Here we directly address how acute fear evolves to anxiety using high field MR in mouse models of PTSD, applying systems-wide longitudinal manganese-enhanced magnetic resonance imaging (MEMRI) to image whole brain responses to unconditioned fear, predator stress (PS), and progression or resolution over time. We report that serotonin transporter knock-out results in sustained anxiety-like behavior and altered neural activity after predator stress. Automated segmentation of SPM maps identifies m regions correlated with progression to PTSD for the first time.

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