Abstract #2305

Tracking adoptive cell transfer of primary human and mouse T-cells in naïve NSG and Balb/c mice, respectively, using PET and MRI methods

Naomi S. Sta Maria¹, Leslie A. Khawli², Sharon W. Lin¹, Vyshnavi Pachipulusu², Long Zhen², Daniel Cohrs³, Alan L. Epstein², and Russell E. Jacobs¹

¹Zilkha Neurogenetic Institute, Keck School of Medicine of USC, Los Angeles, CA, United States, ²Pathology and Laboratory Medicine, Keck School of Medicine of USC, Los Angeles, CA, United States, ³Keck School of Medicine of USC, Los Angeles, CA, United States

We determined the biodistribution of adoptively transferred primary T-cells in naïve mice to create a basis for quantifying adoptively transferred modified T-cells in mice cancer models, using PET and MRI cell labeling techniques simultaneously. Separate populations of T-cells were labeled with either a PET (89Zr) or an MRI label (ferumoxytol), and were injected intravenously into mice together. Animals underwent simultaneous PET/MRI imaging up to 5 days following cell injection in an MR Solutions 7 Tesla scanner with a PET camera. We looked at two models: primary mouse T-cells in naïve Balb/c mice and primary human T-cells in naïve NSG.

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