Abstract #1212

Longitudinal assessment of relaxation and magnetization transfer saturation rates during formalin fixation across fiber pathways of the human brain

Tobias Streubel^1,2, Mohammad Ashtarayeh¹, Herbert Mushumba³, Sebastian Papazoglou¹, Klaus Püschel³, and Siawoosh Mohammadi^1,2

¹Department of Systems Neurosciences, University Medical Center Hamburg-Eppendorf, Hamburg, Germany, ²Department of Neurophysics, Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig, Germany, ³Department of Legal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

We longitudinally investigated the effect of brain tissue fixation, using 4% paraformaldehyde, on three potential quantitative myelin MRI markers across different white matter fiber pathways of the human brain: longitudinal (R₁) and apparent transverse (R₂*) relaxation rates and magnetization transfer (MT) using the quantitative multi-parameter mapping (MPM) protocol. To better understand the temporal evolution of the fixation process within the whole brain and its influence on MRI parameters, we monitored the temporal evolution of the fixation process of a whole human post-mortem brain using the same MPM protocol at 15 time-points (one unfixed, in-situ MRI scan and 14 ex-vivo MRI scans) at different stages of the fixation process (days 1-93).

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