Abstract #1205

Recombinant expression and synthesis of a targeted human contrast agent for quantitative renal MRI

Kasey C Emoto¹, Edwin Baldelomar², Maria Veronica Clavijo-Jordan³, Jennifer R Charlton⁴, Courtnie Yokono⁵, and Kevin M Bennett⁶

¹Biology, University of Hawaii at Manoa, Honolulu, HI, United States, ²Physics, University of Hawaii at Manoa, Honolulu, HI, United States, ³Martinos Center for Biomedical Imaging, Harvard University, Charlestown, MA, United States, ⁴Pediatrics, University of Virginia, Charlottesville, VA, United States, ⁵Bioengineering, University of Hawaii at Manoa, Honolulu, HI, United States, ⁶Washington University in St. Louis, Clayton, MO, United States

This study demonstrates the synthesis and use of a human recombinant cationic ferritin nanoparticle, synthesized in ecoli, as contrast agent for targeted renal imaging. Injected nanoparticles accumulated in the glomerular basement membrane in a mouse, allowing measurement of nephron endowment using gradient echo imaging and automated segmentation. Use of human recombinant contrast agents may allow improved biocompatibility for clinical translation.

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