Abstract #0203

Spanning spatiotemporal scales with simultaneous mesoscopic calcium imaging and functional MRI

Evelyn MR Lake¹, Xinxin Ge², Xilin Shen¹, Peter Herman¹, Fahmeed Hyder^1,3, Jessica A Cardin^4,5, Michael J Higley^4,5,6,7, Dustin Scheinost^1,8,9, Xenophon Papademetris^1,3, Michael C Crair^2,5,10, and R Todd Constable^1,11,12

¹Department of Radiology and Biomedical Imaging, Yale University, New Haven, CT, United States, ²Department of Neurobiology, Yale University, New Haven, CT, United States, ³Department of Biomedical Engineering, Yale University, New Haven, CT, United States, ⁴Department of Neuroscience, Yale University, New Haven, CT, United States, ⁵Kavli Institute for Neuroscience, Yale University, New Haven, CT, United States, ⁶Program in Cellular Neuroscience, Yale University, New Haven, CT, United States, ⁷Neurodegeneration and Repair, Yale University, New Haven, CT, United States, ⁸Department of Statistics and Data Science, Yale University, New Haven, CT, United States, ⁹The Child Study Center, Yale University, New Haven, CT, United States, ¹⁰Department of Ophthalmology and Visual Science, Yale University, New Haven, CT, United States, ¹¹Department of Neurosurgery, Yale University, New Haven, CT, United States, ¹²Interdepartmental Neuroscience Program, Yale University, New Haven, CT, United States

Neuroscience interrogates brain function across multiple spatiotemporal scales. Yet, most research is confined to one spatiotemporal milieu limiting translation of knowledge across scales. Here we span spatiotemporal scales having built a custom apparatus and analytical framework for simultaneous wide field mesoscopic Ca²⁺ imaging of the entire cortex and fMRI at 11.7T in mice. We describe the new hardware/software, and present three findings: there is correspondence between spontaneous fluctuations in the magnitude of Ca²⁺ and fMRI evoked responses, Ca²⁺ and fMRI connectivity metrics are stable throughout an imaging session, and there is correspondence between Ca²⁺ and fMRI spontaneous activity patterns.

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