Spatially localized pure shift 1H MRS for biological tissues at 7 T

Proton magnetic resonance spectroscopy (¹H MRS) presents an effective tool for in vivo studies on biological tissues by the non-invasive detection manner. However, due to limited proton chemical shift range and extensive J coupling splittings, spectral congestions or even overlapping are generally encountered in resulting 1D ¹H MRS acquired by routine STimulated Echo Acquisition Mode (STEAM) and Point resolved spectroscopy (PRESS) experiments. In this report, we presents a previously-unreported MAS approach to obtain spatially localized 1D pure shift spectra with spectral simplification, potentially useful for studies on biological samples.