Abstract #3066

Assessing γ-glutamyl transpeptidase activity in kidney using hyperpolarized γ-Glu-[1-13C]Gly

Steffen F. Frank¹, Hikari A. I. Yoshihara¹, Marino Itoda², Shinsuke Sando², and Rolf Gruetter^3,4,5

¹Laboratory for Functional and Metabolic Imaging, EPFL, Lausanne, Switzerland, ²Department of Chemistry and Biotechnology, The University of Tokyo, Tokyo, Japan, ³Department of Radiology, University of Geneva, Geneva, Switzerland, ⁴Department of Radiology, University of Lausanne, Lausanne, Switzerland, ⁵Centre for Biomedical Imaging, EPFL, Lausanne, Switzerland

Hyperpolarized γ-Glu-[1-¹³C]Gly provides a non-invasive means to detect γ-glutamyl transpeptidase (GGT) enzyme activity in vivo and indicates its potential for application in functional imaging. Since GGT is most abundant in the proximal tubules of the kidney, and since the properties of γ-Glu-[1-¹³C]Gly are suitable for in vivo hyperpolarized ¹³C metabolic analysis, it was proposed as a molecular probe to study kidney function. The aim of the present study is to identify the dose of γ-Glu-[1-¹³C]Gly that gives high NMR sensitivity in the unsaturated state of the GGT enzyme.

This abstract and the presentation materials are available to members only; a login is required.

Join Here