Abstract #1551

Integrating Magnetic Resonance Imaging with Live Lung Intravital Microscopy: A Novel Platform to Evaluate the Effect of Radiation on Lung Tumors

Shampa Chatterjee¹, Luis Loza², Mehrdad Pourfathi², Sarmad Siddiqui², Jian Tao¹, Harrilla Profka², Ian Duncan², Hooman Hamedani², Kai Ruppert², Diane Lim³, Yan Liu³, Jose Conejo-Garcia⁴, Mary Spencer², Tahmina Achekzai², Stephen Kadlecek², and Rahim R. Rizi²

¹Physiology, University of Pennsylvania, Philadelphia, PA, United States, ²Radiology, University of Pennsylvania, Philadelphia, PA, United States, ³Sleep Medicine, University of Pennsylvania, Philadelphia, PA, United States, ⁴Moffitt Cancer Center, Tampa, FL, United States

We propose that, when used in combination with MRI imaging, live lung intravital fluorescence microscopy can be a powerful tool for detecting the effects of radiotherapy on lung tumors. In this study, we monitored pulmonary nodules pre- and post-radiation in a novel murine model (Kras(G12D)/p53^fl/fl/myr-p110) with tumor regulation by Cre-recombinase. Using the reporter gene EGFP fluorescence, a significant loss of the tumor was observed post-radiation, which correlated with reduced fluorescent signal from the same region of the lung.

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