Abstract #5626

Monitoring of cell proliferation by 19F-MRS via quantitation of 3'-deoxy-3'-fluorothymidine (FLT) and its monophosphate metabolite (FLT-MP) in invivo

Inok Ko¹, Ki-Hye Jung¹, Kyung Jun Kang¹, Kyo Chul Lee¹, Yong Jin Lee¹, Jung-Young Kim¹, Sang Moo Lim², and Ji-Ae Park¹

¹Division of RI-Convergence Research, Korea Institute Radiological and Medical Sciences, Seoul, Korea, Republic of, ²Department of Nuclear Medicine, Korea Institute Radiological and Medical Sciences, Seoul, Korea, Republic of

Distinguishing between FLT(fluorothymidine) and FLT-MP(flruorothymidine - monophosphate) by imaging methods is important for evaluating the tumor cell proliferation rate. The aim of this study is to develop and validate a suitable ¹⁹F MR Spectroscopy for measuring TK1 activity via quantitation of FLT and FLT-MP in vivo. We observed the good correlations between SNR and FLT concentration (r2= 0.94). In phantom study, the locations of FLT and FLT-MP was -175.99, -175.24 ppm, respectively. In vivo study, FLT spectrum in mouse tumor was observed in 25 min after injection, whereas FLT-MP spectrum occurred in 90 min after injection. This result shows that ¹⁹F MR Spectroscopy is suitable for monitoring of FLT-MP generation in in vivo.

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