Abstract #3686

Investigation of Brain Tissue Fixation on Iron Concentration, Magnetic Susceptibility and Effective Transverse Relaxation Rate

Andreas Deistung^1,2, Verena Endmayr³, Simon Hametner³, Max Prihoda^4,5, Xiang Feng¹, Hans Lassmann³, Jürgen Rainer Reichenbach¹, Simon Daniel Robinson⁶, Evelin Haimburger⁴, Christian Menard⁷, Thomas Haider⁸, Hannes Traxler⁹, Siegfried Trattnig⁶, and Günther Grabner^4,5,6

¹Medical Physics Group, Institute for Diagnostic and Interventional Radiology, Jena University Hospital – Friedrich Schiller-University, Jena, Germany, ²Section of Experimental Neurology, Department of Neurology, Essen University Hospital, Essen, Germany, ³Center for Brain Research, Medical University of Vienna, Vienna, Austria, ⁴Department of Radiologic Technology, Carinthia University of Applied Sciences, Klagenfurt, Austria, ⁵Institute for Applied Research on Ageing, Carinthia University of Applied Sciences, Klagenfurt, Austria, ⁶High Field Magnetic Resonance Centre, Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna, Vienna, Austria, ⁷Department of Medical Engineering, Carinthia University of Applied Sciences, Klagenfurt, Austria, ⁸University Clinic for Trauma Surgery, Medical University of Vienna, Vienna, Austria, ⁹Center of Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria

We investigated the effect of brain tissue fixation on iron concentration and on quantitative maps of the magnetic susceptibility and effective transverse relaxation rate (R2*). Both R2* and susceptibility distributions of unfixed and fixed tissue were found to be similar, indicating similar underlying proportions of iron across tissues. Quantitatively, however, severe decreases of iron concentration of 25% in putamen and about 15% in white matter were measured after tissue fixation. The iron concentration change due to tissue fixation was captured by R2* and susceptibility which needs to be considered when directly comparing experiments performed with unfixed and fixed brain tissue.

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