Blockface imaging can improve atlases of the rodent brain by supplying high resolution images. This study compares three different contrast stretching methods for enhancing the information in blockface images together with a registration to a 16.4 T atlas of the mouse brain. Contrast enhancement technique used was histogram equalization, adjusting the image intensity values by stretching them between the bottom 1% and the top 1% and CLAHE with a clip limit of 0.01 and a uniform histogram. Registrations was rigid, affine and SyN. By using CLAHE as contrast stretching method a high similarity to the MRI was found.
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