Abstract #0730

19F-perfluorocarbon-labeled human peripheral blood mononuclear cells can be detected in vivo using clinical MRI parameters in a therapeutic cell setting

Corby Fink¹, Jeffrey Gaudet², Matthew S Fox³, Shashank Bhatt⁴, Sowmya Viswanathan⁴, Michael Smith¹, Joseph Chin⁵, Paula Foster², and Gregory Dekaban¹

¹Microbiology and Immunology, Robarts Research Institute, London, ON, Canada, ²Medical Biophysics, Robarts Research Institute, London, ON, Canada, ³Medical Biophysics, Lawson Health Sciences Centre, London, ON, Canada, ⁴Cell Therapy Program, Krembil Research Institute, Toronto, ON, Canada, ⁵Urology, University of Western Ontario, London, ON, Canada

A major hurdle in advancing cell-based cancer vaccines is the inability to track where therapeutic cells migrate post-injection. We present the first study conducted in which primary Good Manufacturing Practice-grade ¹⁹Fluorine (¹⁹F) labeled all peripheral blood mononuclear cell lineages (PBMC) and ¹⁹F cellular MRI was used to track and quantify in vivo migration in a mouse model. Secondly, we present the highest sensitivity for ¹⁹F detection reported in the literature and the first time a 1.2cm deep injection of ¹⁹F-labeled PBMC using a small dual-tuned surface coil has been detected using a clinical scanner and MR protocol.

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