Abstract #2042
Combined optogenetic fMRI and optical Ca2+-recordings for functional mapping of thalamo-cortical circuits in rat
Lydia Wachsmuth 1 , Florian Schmid 1 , Miriam Schwalm 2 , Albrecht Stroh 2 , and Cornelius Faber 1
1
Department of Clinical Radiology, University
of Mnster, Mnster, Germany,
2
Institute
of Microscopic Anatomy and Neurobiology, Johannes
Gutenberg-University Mainz, Mainz, Germany
We combined small animal fMRI with fiber-based
optogenetic excitation of CHR2 and C1V1, transduced in
rat cortex and thalamus, and with cortical fluorescence
detection of a Ca2+-sensitive dye (OGB-1) in order to
assess the scale of optogenetic network activation in
terms of the spatial and temporal patterns of network
recruitment. Onset, peak time and decay characteristics
of BOLD time courses and amplitude and decay
characteristic of Ca2+ responses appeared similar upon
optogenetic and sensory-driven, endogenous network
activation, confirming that ofMRI can serve as a model
for functional mapping of brain circuits.
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