Abstract #0319
Resonance frequency-shifting nitroxide for probing proteolytic activity in vivo using the Overhauser-enhanced MRI technique
Neha KOONJOO 1 , Grard Audran 2 , Lionel Bosco 2 , Paul Brmond 2 , Elodie Parzy 1 , Philippe Massot 1 , Matthieu Lepetit-Coiff 1,3 , Jean-Michel Franconi 1 , Sylvain R.A Marque 2 , Eric Thiaudire 1 , and Philippe Mellet 1,4
1
Centre de Rsonance Magntique des Systmes
Biologiques, Bordeaux, France, Metropolitan,
2
UMR
7273 Aix-Marseille Universit, Marseille, France,
Metropolitan,
3
Siemens,
Saint-Denis, France, Metropolitan,
4
INSERM,
Universit de Bordeaux Segalen, Bordeaux, France,
Metropolitan
Proteolysis of a line-shifting nitroxide-OMRI probe was
visualized in the digestive tract of living mice using
Overhauser-enhanced MRI. Upon enzymatic action on the
probe, its hyperfine coupling constants are shifted;
thus resulting in two detectable electronic excitation
frequencies enabling both substrate and end product
visualization. High signal enhancements revealed either
the substrate or its end product in the stomach. Highly
resolved 3D-keyhole OMRI images of resolution 0.5mm
3
were
obtained with a fully balanced steady state sequence
TrueFISP at 0.2T in 18 seconds. This work is a
steppingstone in
in
vivo
pathology-related
proteolysis detection.
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