Abstract #4096
Absolute quantitation of metabolites in human prostate cancer biopsies by HR-MAS 1 H NMR spectroscopy
Basetti Madhu 1 , Greg Shaw 1 , David Neal 1 , and John R Griffiths 1
1
Cancer Research UK Cambridge Institute,
University of Cambridge, Cambridge, England, United
Kingdom
Human prostate biopsies were analysed with HRMAS
1
H
NMR spectroscopy. Absolute metabolite concentrations of
the following metabolites were estimated by a modified
LC-Model basis set in benign, low grade and high grade
prostate cancer samples: alanine, lactate, glutamine,
glutamate, citrate, choline, phosphocholine (PC)+
glycerophosphocholine(GPC), creatine + phosphocreatine,
taurine, myo-inositol and spermine . Alanine was
significantly elevated in both low and high-grade
prostate cancer biopsies; total choline(choline+PC+GPC)
and glutamate were significantly higher in high-grade
malignant prostate. None of the macromolecule (0.9ppm,
2.0ppm) and lipid signals (0.9ppm, 1.3ppm and 2.0ppm)
showed any statistical differences between the benign
and cancer samples
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