Abstract #3927
Combination of a 13 C cryoprobe with hyperpolarization allows real time observation of pyruvate carboxylation in the perfused mouse heart
Colin Purmal 1 , Blanka Kucejova 1 , Shawn Burgess 1 , Craig Malloy 1 , Dean Sherry 1 , and Matthew E Merritt 1
1
AIRC, UTSW Medical Center, Dallas, TX,
United States
Murine models of myocardial metabolism are a pervasive
tool used by the cardiovascular research community.
Development of methods for monitoring energy metabolism
in the perfused mouse heart would augment the
understanding of a variety of myocardial pathologies and
dysfunctions. Here, hyperpolarized pyruvate is combined
with a
13
C
optimized cryogenic probe to produce an approximate
sensitivity gain of 140,000x for carbon spectroscopy.
The resulting spectra in the functioning heart allow
pyruvate carboxylation to be monitored in real time, a
pathway accepted to have a relative activity of about 5
% of that of pyruvate dehydrogenase.
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