Abstract #3811
Pyruvate Decarboxylase as a Reporter Gene for Magnetic Resonance Spectroscopic Imaging (MRSI)
Piotr Dzien 1 , Sui-Seng Tee 1 , Mikko Kettunen 1 , Timothy Larkin 1 , Scott Lyons 1 , Kerstin Timm 1 , De-En Hu 1 , Tiago Rodrigues 1 , Eva Serrao 1 , Elizabeth Mannion 1 , Paula D'Santos 1 , and Kevin Brindle 1
1
Biochemistry, University of Cambridge,
Cambridge, Cambridgeshire, United Kingdom
A reporter gene that could be used with MRI would allow
exploitation of the advantages of this imaging technique
- spatial and temporal resolution and the penetration
depth necessary for in vivo imaging. DNP increases the
signal-to-noise ratio sufficiently to allow direct
detection of the 13C tracer in real time and presents a
novel approach for developing MR reporter genes. Here we
report a proof of concept study in which we use
inducible expression of a bacterial gene encoding the
enzyme, pyruvate decarboxylase (PDC), as a marker for
DNP MRSI.
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