Abstract #2971
Exploring Energy Metabolism in Zebrafish Brain: A 1 H-[ 13 C]-NMR Study
Anant Bahadur Patel 1 , Madhavi Adusmilli 1 , Sandeep Saxena 1 , and Mohammed M Idris 1
1
CSIR-Centre for Cellular and Molecular
Biology, Hyderabad, Andhra Pradesh, India
Brain metabolism was investigated in zebrafish by
1
H-[
13
C]-NMR
spectroscopy. Zebrafish were anesthetized with tricane
and [1,6-
13
C
2
]glucose was
administered through intracardiac injection. The
concentration and
13
C
labeling of brain metabolites were measured using
1
H-[
13
C]-NMR
spectroscopy in brain extract. The
13
C
turnover of brain amino acids from [1,6-
13
C
2
]glucose
in zebrafish is similar to the pattern observed in rat
and mouse brain. Moreover, the brain energy metabolism
in zebrafish is decreased with acute ethanol treatment.
Zebrafish would be useful alternate model to study
mechanism of various neurological disorders and
screening of drugs.
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