Abstract #2863
Peptide-based MRI contrast agent and near-infrared fluorescent probe for intratumoral legumain detection
Gin-Chung Liu 1 , Yu-Jen Chen 2 , Chia-Yun Chen 1 , Shou-Cheng Wu 2 , and Yun-Ming Wang 2
1
Department of Medical Imaging, Kaohsiung
Medical University Hospital, Kaohsiung Medical
University, Kaohsiung, Taiwan,
2
Department
of Biological Science and Technology, National Chiao
Tung University, HsinChu, Taiwan
In this study, we developed a MRI contrast agent
([Gd-NBCB-TTDA-Leg(L)]) and a NIR fluorescence (NIRF)
probe (CyTE777-Leg(L)-CyTE807) to monitor legumain
activity in tumors. The MRI contrast agent was prepared
by introduction of cyclobutyl and benzyl group residues
to TTDA, followed by a legumain-specific substrate
peptide (Leg(L)). The NIRF probe was designed by
conjugating two fluorochromes with Leg(L). Peptide
cleavage of the MRI contrast agent by legumain can
increase its hydrophobicity and promote contrast
enhancements. Similar cleavage effect on NIR probes
relieves the self quench of the probe.
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