Abstract #2822
Combining fMRI with optical Ca 2+ recordings to define the impact of brain states on BOLD
Miriam Schwalm 1 , Lydia Wachsmuth 2 , Florian Schmid 2 , Cornelius Faber 2 , and Albrecht Stroh 1
1
Institute of Microscopic Anatomy &
Neurobiology - Research Group Molecular Imaging &
Optogenetics, Johannes Gutenberg-University Mainz,
Mainz, Rheinland-Pfalz, Germany,
2
Department
of Clinical Radiology, University of Mnster,
Nordrhein-Westfalen, Germany
Revealing the neuronal underpinnings of fMRI requires
monitoring of suprathreshold spiking. We implemented the
combination of optical Ca2+ recordings with BOLD fMRI in
rat somatosensory cortex and recorded Ca2+ and BOLD
signals in two brain states: in Up/Down state, sensory
stimulation did not lead to BOLD activation, despite
initiation of Ca2+ waves. In persistent Up state, we
recorded primary neuronal Ca2+ responses, and BOLD
activation. The duration of the BOLD response depended
on the duration of stimulus trains, even though Ca2+
transients revealed adaptation of the neuronal response.
Our data suggests that neuronal spiking plays a minor
role in BOLD.
This abstract and the presentation materials are available to members only;
a login is required.
Join Here
