Abstract #2814
Development and evaluation of labeled islet cells using iron oxide nanoparticles and DNA hybridization
Ryusuke Nakai 1,2 , Narufumi Kitamura 1 , and Hiroo Iwata 1
1
Institute for Frontier Medical Sciences,
Kyoto University, Kyoto, Japan,
2
Kokoro
Research Center, Kyoto University, Kyoto, Japan
In this study, we developed a labeling method for islet
cells with superparamagnetic iron oxide particles
(SPIOs) based on DNA hybridization, which has been
successfully applied to cell surface modification. The
labeling efficiency of this method was evaluated by MRI.
The SPIO-labeled islet cells could be clearly detected
as dark spots by T2*-weighted MR image, whereas
non-labeled islet cells could not be detected. The
method is also likely to be applicable for use with
other cells, such as stem cells, and for labeling at
multiple sites by changing the DNA sequence.
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